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primary rabbit monoclonal antibody against ace2  (Thermo Fisher)


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    Structured Review

    Thermo Fisher primary rabbit monoclonal antibody against ace2
    <t>ACE2</t> expression in human arterial endothelial cells upon inflammatory activation. Human arterial endothelial cells (HAECs) were treated for different periods of time with TNFα (50 ng/mL) or IL-1β (10 ng/mL), followed by RNA extraction. ( A ) RNA was reversed transcribed, and ACE2 mRNA was measured via quantitative PCR (mean ± SEM, n = 3, ANOVA was performed with GraphPad Prism 6.0 with Fisher’s LSD test between control and treated samples; p -values as indicated). ( B ) ACE2 protein levels were determined in HAECs treated as indicated by immunofluorescence staining and normalization of the staining intensity to the DNA staining (mean values ± SEM, n = 3; ANOVA and Fisher’s LSD test between treated samples and untreated control; * p < 0.05, ** p < 0.01, *** p < 0.0001).
    Primary Rabbit Monoclonal Antibody Against Ace2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+rabbit+monoclonal+antibody+against+ace2/pmc11083315-84-6-13
    Average 90 stars, based on 1 article reviews
    primary rabbit monoclonal antibody against ace2 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Effects of Chronic Inflammatory Activation of Murine and Human Arterial Endothelial Cells at Normal Lipoprotein and Cholesterol Levels In Vivo and In Vitro"

    Article Title: Effects of Chronic Inflammatory Activation of Murine and Human Arterial Endothelial Cells at Normal Lipoprotein and Cholesterol Levels In Vivo and In Vitro

    Journal: Cells

    doi: 10.3390/cells13090773

    ACE2 expression in human arterial endothelial cells upon inflammatory activation. Human arterial endothelial cells (HAECs) were treated for different periods of time with TNFα (50 ng/mL) or IL-1β (10 ng/mL), followed by RNA extraction. ( A ) RNA was reversed transcribed, and ACE2 mRNA was measured via quantitative PCR (mean ± SEM, n = 3, ANOVA was performed with GraphPad Prism 6.0 with Fisher’s LSD test between control and treated samples; p -values as indicated). ( B ) ACE2 protein levels were determined in HAECs treated as indicated by immunofluorescence staining and normalization of the staining intensity to the DNA staining (mean values ± SEM, n = 3; ANOVA and Fisher’s LSD test between treated samples and untreated control; * p < 0.05, ** p < 0.01, *** p < 0.0001).
    Figure Legend Snippet: ACE2 expression in human arterial endothelial cells upon inflammatory activation. Human arterial endothelial cells (HAECs) were treated for different periods of time with TNFα (50 ng/mL) or IL-1β (10 ng/mL), followed by RNA extraction. ( A ) RNA was reversed transcribed, and ACE2 mRNA was measured via quantitative PCR (mean ± SEM, n = 3, ANOVA was performed with GraphPad Prism 6.0 with Fisher’s LSD test between control and treated samples; p -values as indicated). ( B ) ACE2 protein levels were determined in HAECs treated as indicated by immunofluorescence staining and normalization of the staining intensity to the DNA staining (mean values ± SEM, n = 3; ANOVA and Fisher’s LSD test between treated samples and untreated control; * p < 0.05, ** p < 0.01, *** p < 0.0001).

    Techniques Used: Expressing, Activation Assay, RNA Extraction, Real-time Polymerase Chain Reaction, Immunofluorescence, Staining

    Related Articles

    Staining:

    Article Title: Effects of Chronic Inflammatory Activation of Murine and Human Arterial Endothelial Cells at Normal Lipoprotein and Cholesterol Levels In Vivo and In Vitro
    Article Snippet: HAECs were seeded into gelatin-coated 96-well plates and stimulated with 50 ng/mL TNFα or 10 ng/mL IL-1β for 5 h, 24 h, or 5 d. After the incubation, the cells were fixed with 4% PFA (Sigma-Aldrich) for 10 min. Membrane permeabilization was performed with 0.2% Triton-X 100 (Serva, Heidelberg, Germany) and blocking of unspecific binding sites with 3% goat serum (Abcam). .. The cells were stained with primary rabbit monoclonal antibody against ACE2 (1:100, #MA5-32307, Invitrogen) and goat anti-rabbit Alexa Fluor 647 (1:500, #A21245, Invitrogen) antibody. .. Nuclear counterstaining was performed by using 1 μg/mL Hoechst 33258 (Cayman Chemical, Ann Arbor, MI, USA).



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    primary rabbit monoclonal antibody against ace2  (Thermo Fisher)
    90
    Thermo Fisher primary rabbit monoclonal antibody against ace2
    <t>ACE2</t> expression in human arterial endothelial cells upon inflammatory activation. Human arterial endothelial cells (HAECs) were treated for different periods of time with TNFα (50 ng/mL) or IL-1β (10 ng/mL), followed by RNA extraction. ( A ) RNA was reversed transcribed, and ACE2 mRNA was measured via quantitative PCR (mean ± SEM, n = 3, ANOVA was performed with GraphPad Prism 6.0 with Fisher’s LSD test between control and treated samples; p -values as indicated). ( B ) ACE2 protein levels were determined in HAECs treated as indicated by immunofluorescence staining and normalization of the staining intensity to the DNA staining (mean values ± SEM, n = 3; ANOVA and Fisher’s LSD test between treated samples and untreated control; * p < 0.05, ** p < 0.01, *** p < 0.0001).
    Primary Rabbit Monoclonal Antibody Against Ace2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+rabbit+monoclonal+antibody+against+ace2/pmc11083315-84-6-13
    Average 90 stars, based on 1 article reviews
    primary rabbit monoclonal antibody against ace2 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    rabbit primary monoclonal antibody against ace2  (Santa Cruz Biotechnology)
    90
    Santa Cruz Biotechnology rabbit primary monoclonal antibody against ace2
    <t>ACE2</t> expression in human arterial endothelial cells upon inflammatory activation. Human arterial endothelial cells (HAECs) were treated for different periods of time with TNFα (50 ng/mL) or IL-1β (10 ng/mL), followed by RNA extraction. ( A ) RNA was reversed transcribed, and ACE2 mRNA was measured via quantitative PCR (mean ± SEM, n = 3, ANOVA was performed with GraphPad Prism 6.0 with Fisher’s LSD test between control and treated samples; p -values as indicated). ( B ) ACE2 protein levels were determined in HAECs treated as indicated by immunofluorescence staining and normalization of the staining intensity to the DNA staining (mean values ± SEM, n = 3; ANOVA and Fisher’s LSD test between treated samples and untreated control; * p < 0.05, ** p < 0.01, *** p < 0.0001).
    Rabbit Primary Monoclonal Antibody Against Ace2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+rabbit+monoclonal+antibody+against+ace2/anti+ace2/pm37312182-118-40-47
    Average 90 stars, based on 1 article reviews
    rabbit primary monoclonal antibody against ace2 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ACE2 expression in human arterial endothelial cells upon inflammatory activation. Human arterial endothelial cells (HAECs) were treated for different periods of time with TNFα (50 ng/mL) or IL-1β (10 ng/mL), followed by RNA extraction. ( A ) RNA was reversed transcribed, and ACE2 mRNA was measured via quantitative PCR (mean ± SEM, n = 3, ANOVA was performed with GraphPad Prism 6.0 with Fisher’s LSD test between control and treated samples; p -values as indicated). ( B ) ACE2 protein levels were determined in HAECs treated as indicated by immunofluorescence staining and normalization of the staining intensity to the DNA staining (mean values ± SEM, n = 3; ANOVA and Fisher’s LSD test between treated samples and untreated control; * p < 0.05, ** p < 0.01, *** p < 0.0001).

    Journal: Cells

    Article Title: Effects of Chronic Inflammatory Activation of Murine and Human Arterial Endothelial Cells at Normal Lipoprotein and Cholesterol Levels In Vivo and In Vitro

    doi: 10.3390/cells13090773

    Figure Lengend Snippet: ACE2 expression in human arterial endothelial cells upon inflammatory activation. Human arterial endothelial cells (HAECs) were treated for different periods of time with TNFα (50 ng/mL) or IL-1β (10 ng/mL), followed by RNA extraction. ( A ) RNA was reversed transcribed, and ACE2 mRNA was measured via quantitative PCR (mean ± SEM, n = 3, ANOVA was performed with GraphPad Prism 6.0 with Fisher’s LSD test between control and treated samples; p -values as indicated). ( B ) ACE2 protein levels were determined in HAECs treated as indicated by immunofluorescence staining and normalization of the staining intensity to the DNA staining (mean values ± SEM, n = 3; ANOVA and Fisher’s LSD test between treated samples and untreated control; * p < 0.05, ** p < 0.01, *** p < 0.0001).

    Article Snippet: The cells were stained with primary rabbit monoclonal antibody against ACE2 (1:100, #MA5-32307, Invitrogen) and goat anti-rabbit Alexa Fluor 647 (1:500, #A21245, Invitrogen) antibody.

    Techniques: Expressing, Activation Assay, RNA Extraction, Real-time Polymerase Chain Reaction, Immunofluorescence, Staining